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Science 3 October 1975:
Vol. 190. no. 4209, pp. 61 - 63
DOI: 10.1126/science.52193
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Articles

Science, Vol 190, Issue 4209, 61-63
Copyright © 1975 by American Association for the Advancement of Science

articles

Differential fluorescent staining of human chromosomes with daunomycin and adriamycin--the d-bands

CC Lin and JH Van de Sande

Human chromosome preparations were treated with a group of anthracycline antibiotics. Well-defined, orange-red fluorescent bands were observed on chromosomes after the slide was stained with daunomycin and adriamycin but not with nogalamycin. The characteristic differential bands appeared to be similar to the banding patterns obtained by the quinacrine techniques. Interaction of these antibiotics with DNA could provide information on the general mechanism of fluorescent banding. Further, these bands (D-bands) appeared to be more stable than the Q-bands and may have some usefulness for routine clinical cytogenetic analysis.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Reverse banding on chromosomes produced by a guanosine-cytosine specific DNA binding antibiotic: olivomycin.
J. van de Sande, C. Lin, and K. Jorgenson (1977)
Science 195, 400-402
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