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Science 4 April 1975:
Vol. 188. no. 4183, pp. 66 - 67
DOI: 10.1126/science.803714
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Articles

Science, Vol 188, Issue 4183, 66-67
Copyright © 1975 by American Association for the Advancement of Science

articles

Reconstitution of Rh (D) antigen activity from human erythrocyte membranes solubilized by deoxycholate

DJ Lorusso and FA Green

Proteins were selectively solubilized from human erythrocyte membranes with deoxycholate. After ultracentrifugation and preliminary fractionation procedures, the detergent was removed from the soluble membranes by Bio-Beads SM-2 and dialysis against 5 millimolar magnesium chloride. Reaggregation took place with the apparent formation of vesicles which showed serologically specific Rh antigen activity.




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Science. ISSN 0036-8075 (print), 1095-9203 (online)