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Science 9 August 1974:
Vol. 185. no. 4150, pp. 531 - 533
DOI: 10.1126/science.185.4150.531
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Articles

Chloral Hydrate: A Solvent for Biological Membranes

Byron Ballou 1, G. Sundharadas 1, and Marilyn L. Bach 1

1 Immunobiology Research Center and Departments of Medical Genetics, Medical Microbiology, Pediatrics, and Pharmacology, University of Wisconsin, Madison 53706

A buffer system conitainin chloral hydrate, taurine, and bromopyridinium lactate was used to dissolve several biological membranes and separate their protein components by polyacrylamide gel electrophoresis. This solvent system was capable of separating molecules of similar size on the hasis of their charge and allows easy recovery of the proteins Thus. aqueous chloral hydrate is an effective solvent for biological membranes.


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