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Science 21 June 1974:
Vol. 184. no. 4143, pp. 1297 - 1298
DOI: 10.1126/science.184.4143.1297
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Articles

Cell-Cycle Analysis in 20 Minutes

Harry A. Crissman 1 and Robert A. Tobey 1

1 Biophysics and Instrumentation Group and Cellular and Molecular Radiobiology Group, Los Alamos Scientific Laboratory, University of California, Los Alamos, New Mexico 87544

Mithramycin added to mammalian cells fixed in aqueous ethanol is bound to DNA and fluoresces in direct proportion to the cellular DNA content. Quantitative fluorescence measurement by means of a high-speed flow system instrument provides a rapid method for cell-cycle analysis and for the first time permits continuous monitoring of cell-cycle kinetics during ongoing experiments.


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