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Science 7 June 1974:
Vol. 184. no. 4141, pp. 1093 - 1096
DOI: 10.1126/science.184.4141.1093
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Articles

Banded Marker Chromosomes as Indicators of Intraspecies Cellular Contamination

Walter A. Nelson-Rees 1, Robert R. Flandermeyer 1, and Paula K. Hawthorne 1

1 Cell Culture Laboratory, University of California, School of Public Health, Naval Biomedical Research Laboratory, Oakland 94625

Chromosome banding revealed marked chromosomes characteristic of HeLa cells in cultures designated HEK, HEK/HRV, HBT-3, HBT-39B, MA160, and a strain of SA-4TxS-Husa1. Ohter HeLa cell characteristics found were glucose-6-phosphate dehydrogense type A mobility and lack the Y chromosome. Conventional chromosome analysis and immunological and enzymatic technique serve to monitor species specificity and racial origin of the donor. Chromosome banding, however, can monitor intralinear karyotype peculiarity and its evolution during long-term cultivation.


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