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Science 31 May 1974:
Vol. 184. no. 4140, pp. 992 - 994
DOI: 10.1126/science.184.4140.992
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Articles

Enzyme Measurements on Single Cells

Linda Wudl 1 and Kenneth Paigen 1

1 Department of Molecular Biology, Roswell Park Memorial Institute, Buffalo, New York 14203

A simple and sensitive procedure has been developed for enzymatic assays in single cells and applied to the measurement of beta-glucuronidase in single parenchymal cells of liver. Cells deposited in microdroplets under oil were ruptured by freezing and thawing and allowed to react with a fluorogenic substrate. The fluorescence is developed by diffusing an organic base into the droplets and measured in a fluorescence microscope equipped with a photomultiplier.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)