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Science 8 March 1974:
Vol. 183. no. 4128, pp. 964 - 965
DOI: 10.1126/science.183.4128.964
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Articles

Choline Content of Rat Brain

W. B. Stavinoha 1 and S. T. Weintraub 1

1 Department of Pharmacology, University of Texas Health Science Center, San Antonio 78284

The free choline concentration in the rat brain was found to be 26.3 nanomoles per gram of brain tissue. This value was obtained through use of 6-second microwave irradiation for killing animals and inactivating enzymes, followed by a pyrolysis-gas chromatographic assay procedure. The identities of compounds measured from brain samples were verified by mass spectrometry.


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