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Science 15 February 1974:
Vol. 183. no. 4125, pp. 656 - 657
DOI: 10.1126/science.183.4125.656
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Articles

Cholera Toxin: Interaction of Subunits with Ganglioside GM1

Simon van Heyningen 1

1 Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, Great Britain

Vibrio cholerae exotoxin is an aggregate of two different noninterconvertible subunits (molecular weights about 15,000 and about 25,000). Only the smaller subunit reacts with ganglioside GM1, a possible biological receptor. The larger subunit, found only in active toxin molecules, can be eluted with 8 molar urea from insoluble complexes of toxin, ganglioside, and cerebroside.


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