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Science 25 January 1974:
Vol. 183. no. 4122, pp. 334 - 336
DOI: 10.1126/science.183.4122.334
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Articles

Detection of Heat-Labile Escherichia coli Enterotoxin with the Use of Adrenal Cells in Tissue Culture

Sam T. Donta 1, Harley W. Moon 2, and Shannon C. Whipp 2

1 Department of Medicine, University of Iowa Hospital and Veterans Administration Hospital, Iowa City 52240
2 National Animal Disease Laboratory, Ames, Iowa 50010

Cell-free culture filtrates of heat-labile enterotoxin-producing strains of Escherichia coli are capable of inducing morphological changes and steroidogenesis in monolayer cultures of adrenal cells. These tissue culture changes are simiar to those induced by cholera enterotoxin and cannot be effected by culture filtrates of other enterotoxigenic or enteropathogenic types of bacteria. The results of the tissue culture studies correlated well with those done in the standard intestinal-loop systems and suggest that this tissue culture system could be used to significantly aid epidemiological and molecular studies with heat-labile Escherichia coli enterotoxin.


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