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Science 14 December 1973:
Vol. 182. no. 4117, pp. 1149 - 1151
DOI: 10.1126/science.182.4117.1149
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Articles

Von Willebrand Factor: Dissociation from Antihemophilic Factor Procoagulant Activity

Harvey J. Weiss 1 and Leon W. Hoyer 2

1 Department of Medicine, Roosevelt Hospital, New York 10019, and Columbia University College of Physicians and Surgeons, New York
2 Departmlent of Medicine, University of Connecticut School of Medicine, Farmington 06032

Factor VIII corrects both the clotting defect in hemophilia A and an abnormality of platelet aggregation in von Willebrand's disease. These two activities of factor VIII (antihemophilic factor and von Willebrand factor) are both detected in the void volume when human plasma or cryoprecipitate is chromatographed on Bio-Gel 5M under conditions of isotonic salt concentration. In contrast, antihemophilic factor procoagulant activity is detected with proteins of lower molecular weight when the chromatography is performed with a buffer containing 0.8M NaCl. In this way, the two activities of factor VIII can be dissociated. It remains to be determined whether these components are separate molecules associated as a complex of high molecular weight in plasma or whether they are subunits of a complex macromolecule.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
A sequence variation scan of the coagulation factor VIII (FVIII) structural gene and associations with plasma FVIII activity levels.
K. R. Viel, D. K. Machiah, D. M. Warren, M. Khachidze, A. Buil, K. Fernstrom, J. C. Souto, J. M. Peralta, T. Smith, J. Blangero, et al. (2007)
Blood 109, 3713-3724
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Elevated Factor VIII Levels and the Risk of Thrombosis.
P. W. Kamphuisen, J. C. J. Eikenboom, and R. M. Bertina (2001)
Arterioscler Thromb Vasc Biol 21, 731-738
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Factor VIII.
H. R. GRALNICK, B. S. COLLER, N. R. SHULMAN, J. C. ANDERSEN, and M. HILGARTNER (1977)
Ann Intern Med 86, 598-616
   Abstract »    PDF »
Thermal Injury in von Willebrand Disease: Management of Burn Wound Bleeding.
W. A. Andes, J. P. Baron, R. P. Bowman, and F. R. Rickles (1976)
Arch Surg 111, 280-283
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