Probit Transformation: Improved Method for Defining Synchrony of Cell Cultures
William G. Hagar 1 and
Thomas R. Punnett 2
1 Department of Plant Biology, Carnegie Institution of Washington, Stanford, California 94305
2 Department of Biology, Temple University, Philadelphia, Pennsylvania 19122
Cell numbers can be converted to probits that are used to compare the degree and timing of synchronized cell cultures. Data from synchronous cultures of Chlorella pyrenoidosa have been analyzed by this method by means of a readily available computer program. The method can be used with any biological system that generates normal sigmoidal data.