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Science 19 October 1973:
Vol. 182. no. 4109, pp. 291 - 293
DOI: 10.1126/science.182.4109.291
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Articles

Neuronal Properties of Nymphal and Adult Insect Neurosecretory Cells in vitro

K. R. Seshan 1 and Rita Levi-Montalcini 2

1 Department of Biology, Washington University, St. Louis, Missouri 63130
2 Department of Biology, Washington University, and Laboratorio de Biologia Cellulare CNR, Via G. Romagnosi 18/A, 00196 Rome, Italy

Neurosecretory brain cells from late nymphal and adult cockroaches were grown for 1 to 3 months in a chemically defined medium in combined cultures with embryonic organs or oocytes from nymphal and adult specimens. Under these conditions, neurosecretory cells show vigorous axonal growth. Electron dense granules in cell perikarya and axons of 3-month-old cultures are suggestive of neurosecretory activity in vitro.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)