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Science 3 August 1973:
Vol. 181. no. 4098, pp. 453 - 454
DOI: 10.1126/science.181.4098.453
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Articles

Replication of Human Endothelial Cells in Culture

L. James Lewis 1, John C. Hoak 1, Richard D. Maca 1, and Glenna L. Fry 1

1 Department of Medicine, University of Iowa College of Medicine, Iowa City 52242, and Abbott Laboratories, North Chicago, Illinois 60064

Investigative studies dealing with the properties and functions of endothelial cells have been hampered because there has been little or no success in the isolation, growth, and passage of individual cells in large numbers. We have developed a system whereby pure cultures of endothelial cells derived from umbilical veins can be subcultured for at least five serial passages. Many facets of endothelial function and interaction can be evaluated with the use of this new adaptive system of isolation and culture.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Endothelial Cells in Physiology and in the Pathophysiology of Vascular Disorders.
D. B. Cines, E. S. Pollak, C. A. Buck, J. Loscalzo, G. A. Zimmerman, R. P. McEver, J. S. Pober, T. M. Wick, B. A. Konkle, B. S. Schwartz, et al. (1998)
Blood 91, 3527-3561
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Thrombin Induces the Preproendothelin-1 Gene in Endothelial Cells by a Protein Tyrosine Kinase–Linked Mechanism.
T. A. Marsen, M. S. Simonson, and M. J. Dunn (1995)
Circ. Res. 76, 987-995
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