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Science 9 February 1973:
Vol. 179. no. 4073, pp. 585 - 588
DOI: 10.1126/science.179.4073.585
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Articles

Noradrenergic Stimulation of Cyclic Adenosine Monophosphate in Rat Purkinje Neurons: An Immunocytochemical Study

G. R. Siggins 1, E. F. Battenberg 1, B. J. Hoffer 1, F. E. Bloom 1, and A. L. Steiner 2

1 Laboratory of Neuropharmacology, Division of Special Mental Health Research, National Institute of Mental Health, Saint Elizabeths Hospital, Washington, D.C.
2 Department of Medicine, Albany Medical College, Albany, New York

A specific immunofluorescent histochemical method for cyclic adenosine monophosphate was used to study rat cerebellum. After topical treatment with norepinephrine or stimulation of norepinephrine-containing afferents from locus coeruleus, there was a striking increase in the number of Purkinje cells with strong cyclic adenosine monophosphate reactivity. Other putative inhibitory transmitters had no significant effect on staining of Purkinje cells. The results provide the first histochemical support for the hypothesis that cyclic adenosine monophosphate can be generated postsynaptically in central neurons in response to noradrenergic stimuli.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Cyclic Nucleotides, Protein Phosphorylation and Synaptic Function.
K. G. Beam and P. Greengard (1976)
Cold Spring Harb Symp Quant Biol 40, 157-168
   Abstract »    PDF »
Neurotransmitters increase cyclic nucleotides in postganglionic neurons: immunocytochemical demonstration.
J. Kebabian, F. Blood, A. Steiner, and P Greengard (1975)
Science 190, 157-159
   Abstract »    PDF »
Analogs of cyclic adenosine monophosphate: correlation of inhibition of Purkinje Neurons with Protein Kinase Activation.
G. Siggins and S. Henriksen (1975)
Science 189, 559-561
   Abstract »    PDF »
Dopamine receptors in the brain.
L. Iversen (1975)
Science 188, 1084-1089
   PDF »
Cyclic AMP and cyclic GMP may mediate opposite neuronal responses in the rat cerebral cortex.
T. Stone, D. Taylor, and F. Bloom (1975)
Science 187, 845-847
   Abstract »    PDF »
Interpreting the Failures to Confirm the Depression of Cerebellar Purkinje Cells by Cyclic AMP.
F. E. Bloom, G. R. Siggins, and B. J. Hopper (1974)
Science 185, 627-629
   PDF »
Failure to Confirm Cyclic AMP as Second Messenger for Norepinephrine in Rat Cerebellum.
N. Lake and L. M. Jordan (1974)
Science 183, 663-664
   Abstract »    PDF »
Adenosine 3', 5'-Monophosphate in Cerebrospinal Fluid: Effect of Drugs and Neurologic Disease.
H. Cramer, L. K. Y. Ng, and T. N. Chase (1973)
Arch Neurol 29, 197-199
   Abstract »    PDF »


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