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Science 26 January 1973:
Vol. 179. no. 4071, pp. 400 - 402
DOI: 10.1126/science.179.4071.400
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Articles

Isolation and Structure Determination of Blepharismin, a Conjugation Initiating Gamone in the Ciliate Blepharisma

Takashi Kubota 1, Takashi Tokoroyama 1, Yoshiko Tsukuda 2, Hirozo Koyama 2, and Akio Miyake 3

1 Faculty of Science, Osaka City University, Osaka 558, Japan
2 Shionogi Research Laboratory, Shionogi and Co., Ltd., Osaka 553
3 Max-Planck-Institut für Molekulare Genetik, Berlin 33, Germany

One of the gamones (gamone II) which are effective for the induction of conjugation in Blepharisma intermedium has been isolated in a crystalline form and designated as blepharismin. From the result of chemical and spectroscopic investigations, in which x-ray crystallographic analysis was used as a definitive tool, blepharismin has been found to have the structure of calcium 3-(2'-formylamino-5'-hydroxybenzoyl)lactate.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Developmentally and environmentally regulated expression of gamone 1: the trigger molecule for sexual reproduction in Blepharisma japonicum.
M. Sugiura, S. Kawahara, H. Iio, and T. Harumoto (2005)
J. Cell Sci. 118, 2735-2741
   Abstract »    Full Text »    PDF »
Control factor of nuclear cycles in cilate conjugation: cell-to-cell transfer in multicellular complexes.
A Miyake (1975)
Science 189, 53-55
   Abstract »    PDF »
Blepharmone: A Conjugation-Inducing Glycoprotein in the Ciliate Blepharisma.
A. Miyake and J. Beyer (1974)
Science 185, 621-623
   Abstract »    PDF »
Identification, characterization, and complete amino acid sequence of the conjugation-inducing glycoprotein (blepharmone) in the ciliate Blepharismajaponicum.
M. Sugiura and T. Harumoto (2001)
PNAS 98, 14446-14451
   Abstract »    Full Text »    PDF »


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