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Science 19 January 1973:
Vol. 179. no. 4070, pp. 295 - 297
DOI: 10.1126/science.179.4070.295

Articles

Collagen Synthesis in vitro by Embryonic Spinal Cord Epithelium

Robert L. Trelstad 1, Andrew H. Kang 1, Alan M. Cohen 2, and Elizabeth D. Hay 2

1 Developmental Biology Laboratory, Departments of Medicine and Pathology, Massachusetts General Hospital, Boston 02114
2 Department of Anatomy, Harvard Medical School, Boston 02115

Isolated spinal cords from chick embryos (stages 12 to 15) were incubated in vitro with radioactive proline. The proteins synthesized were fractionated by coprecipitation with added carrier collagen, followed by molecularsieve and ion-exchange chromatography. A portion of the isotopically labeled proteins were found to be collagen molecules consisting only of agr1 chains.


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