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Science 19 January 1973:
Vol. 179. no. 4070, pp. 291 - 293
DOI: 10.1126/science.179.4070.291
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Articles

Lambda Phage DNA: Joining of a Chemically Synthesized Cohesive End

Clifford L. Harvey 1, Rosemary Wright 1, and Alexander L. Nussbaum 1

1 Chemical Research Department, Hoffmann-La Roche, Inc. Nutley, New Jersey 07110

The chemically synthesized dodecamer d(pA-G-G-T-C-G-C-C-G-C-C-C) was annealed, and was covalently joined to lambda phage DNA with bacterio-phage T4 ligase. The 5'-end of the dodecamer was joined to a deoxyguanosine residue. Repair with DNA polymerase I established that the position of joining was the left-hand end of lambda DNA. This is the first time that a chemically synthesized oligonucleotide has been covalently joined to a naturally occurring DNA molecule.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)