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Science 22 December 1972:
Vol. 178. no. 4067, pp. 1293 - 1295
DOI: 10.1126/science.178.4067.1293
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Articles

Translation of Bacteriophage Qbeta Messenger RNA in a Murine Krebs 2 Ascites Tumor Cell-Free System

H. Aviv 1, I. Boime 1, B. Loyd 1, and P. Leder 1

1 Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, Bethesda, Maryland 20014

Qbeta is a small bacterial virus whose three genes are encoded in a single-stranded molecule of RNA. This RNA serves directly as the Qbeta message. Here we describe conditions under which RNA corresponding to the coat cistron of this bacterial virus is translated in a system derived from mammalian cells. Translation of the bacterial virus messenger RNA is less effective than that of mammalian globin messenger RNA, but is somewhat enhanced by mild alkali treatment of the messenger. The synthesized product when subjected to electrophoresis migrates with authentic Qbeta coat protein and yields tryptic peptides that correspond to those derived from the Qbeta coat protein.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)