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Science 15 December 1972:
Vol. 178. no. 4066, pp. 1209 - 1210
DOI: 10.1126/science.178.4066.1209
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Articles

Dispersal of Rabbit Lung into Individual Viable Cells: A New Model for the Study of Lung Metabolism

Kenneth G. Gould Jr. 1, John A. Clements 2, Albert L. Jones 2, and James M. Felts 2

1 Department of the Air Force, School of Aerospace Medicine, Brooks Air Force Base, Texas 78235, and Department of Physiology, University of California, San Francisco 94122
2 Cardiovascular Research Institute, Departments of Pediatrics, Medicine, Anatomy, and Physiology, University of California, San Francisco 94122, and Cell Biology Section, Veterans Administration Hospital, San Francisco 94121

This new enzymatic method disperses rabbit lung into morphologically intact, viable individual cells. The scattered cells constitute more than 50 percent of the original tissue. At least 90 percent of the cells exclude trypan blue from the nucleus. The dispersed lung cells consumed 6.2 microliters of oxygen per hour per milligram, dry weight. They incorporated [1-14C]palmitate into lecithin.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Pulmonary Cell Biology.
R. J. MASON and R. G. CRYSTAL (1998)
Am. J. Respir. Crit. Care Med. 157, S72-S81
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Science. ISSN 0036-8075 (print), 1095-9203 (online)