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Science 21 July 1972:
Vol. 177. no. 4045, pp. 279 - 280
DOI: 10.1126/science.177.4045.279
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Articles

Fluorescent Modification of Adenosine 3',5'-Monophosphate: Spectroscopic Properties and Activity in Enzyme Systems

John A. Secrist III 1, Jorge R. Barrio 1, Nelson J. Leonard 1, Carlos Villar-Palasi 2, and Alfred G. Gilman 2

1 School of Chemical Sciences, University of Illinois, Urbana 61801
2 Department of Pharmacology, University of Virginia School of Medicine, Charlottesville 22901

The synthesis of a highly fluorescent analog of adenosine 3',5'-monophosphate, namely, 1,N6-ethenoadenosine 3',5'-monophosphate, has provided a powerful probe for systems involving adenosine 3',5'-monophosphate. The potential utility of this analog is indicated by its long fluorescent lifetime, detectability at low concentration, and relatively long wavelength of excitation (300 nanometers). In protein kinase systems it is a highly acceptable substitute for adenosine 3',5'-monophosphate.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)