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Science 9 June 1972:
Vol. 176. no. 4039, pp. 1147 - 1149
DOI: 10.1126/science.176.4039.1147
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Articles

Cytochalasin B Inhibits Lymphotoxin Production by Antigen-Stimulated Lymphocytes

Masaru Yoshinaga 1, Byron H. Waksman 1, and Stephen E. Malawista 1

1 Departments of Microbiology and Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06510

Lymph node cells of rats sensitized with hen ovalbumin produced lymphotoxin after 6 to 12 hours of exposure to specific antigen. Lymphotoxin was assayed by its cytotoxicity for fibroblasts from syngeneic embryos during a 72-hour incubation. Cytochalasin B inhibited lymphotoxin production, as well as later DNA synthesis, at concentrations (0.1 to 5.0 micrograms per milliliter) comparable to those which affect microfilament function and cell motility in other systems, and this inhibition was reversible. Binding of antigen was not affected.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)