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Science 9 June 1972:
Vol. 176. no. 4039, pp. 1145 - 1146
DOI: 10.1126/science.176.4039.1145
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Articles
Crystalline Fraction I Protein: Preparation in Large Yield
P. H. Chan 1,
Katsuhiro Sakano 1,
Shalini Singh 1, and
S. G. Wildman 1
1 Department of Botanical Sciences and Molecular Biology Institute, University of California, Los Angeles 90024
Abolut 1 milligram of twice-recrystallized fraction I protein of constant specific ribulose diphosphate carboxylase activity per gram of leaves (fresh weight) has been obtained from each of seven different species of Nicotiana and 14 reciprocal, interspecific F1 hybrids. Crystals are produced from honmogenates that have only been centrifuged to remove particulate matter.
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