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Science 19 May 1972:
Vol. 176. no. 4036, pp. 798 - 800
DOI: 10.1126/science.176.4036.798
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Articles

Viral and Cellular DNA Polymerase: Comparison of Activities with Synthetic and Natural RNA Templates

Marjorie S. Robert 1, R. Graham Smith 1, Robert C. Gallo 1, Prem S. Sarin 2, and John W. Abrell 2

1 Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, Maryland 20014
2 Bionetics Research Laboratories, Bethesda, Maryland 20014

Two DNA polymerases purified from normal human lymphocytes are distinguishable from the viral reverse transcriptases of avian myeloblastosis virus and Mason-Pfizer monkey virus by their relative affinity for select templates. In this respect, the activity of the two normal human lymphocyte polymerases closely resembles the activity of Escherichia coli DNA polymerase 1. The viral and cellular DNA polymerases are equally active with the nonspecific template, poly(rA) · poly(dT). Criteria for distinguishing the activity of viral reverse transcriptase are discussed.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Reverse Transcriptase in Normal Rhesus Monkey Placenta.
R. J. Mayer, R. G. Smith, and R. C. Gallo (1974)
Science 185, 864-867
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Human DNA Polymerase III (R-DNA Polymerase): Distinction from DNA Polymerase I and Reverse Transcriptase.
B. J. Lewis, J. W. Abrell, R. G. Smith, and R. C. Gallo (1974)
Science 183, 867-869
   Abstract »    PDF »
Relationships between Components in Primate RNA Tumor Viruses and in the Cytoplasm of Human Leukemic Cells: Implications to Leukemogenesis.
R. C. Gallo, R. E. Gallagher, N. R. Miller, H. Mondal, W. C. Saxinger, R. J. Mayer, R. G. Smith, and D. H. Gillespie (1974)
Cold Spring Harb Symp Quant Biol 39, 933-961
   Abstract »    PDF »
DNA Polymerases from RNA Tumor Viruses and Human Cells: Inhibition by Polyuridylic Acid.
J. W. Abrell, R. G. Smith, M. S. Robert, and R. C. Gallo (1972)
Science 177, 1111-1114
   Abstract »    PDF »
Reverse Transcriptases of Primate Viruses as Immunological Markers.
E. M. Scolnick, W. P. Parks, and G. J. Todaro (1972)
Science 177, 1119-1121
   Abstract »    PDF »


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