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Science 10 March 1972:
Vol. 175. no. 4026, pp. 1116 - 1117
DOI: 10.1126/science.175.4026.1116
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Articles

Antigenic Relationships in Mammalian DNA Polymerase

Lucy M. S. Chang 1 and F. J. Bollum 1

1 Departments of Biochemistry and Oral Biology, University of Kentucky Medical Center, Lexington 40506

Rabbit antibody was prepared against a high-molecular-weight DNA polymerase purified from the soluble fraction of calf thymus gland. This antibody does not inhibit terminal deoxynucleotidyl transferase isolated from that source, but does inhibit both low-molecular-weight and high-molecular-weight DNA polymerases isolated from cytoplasmic and nuclear fractions of a number of mammalian tissues (mouse L cells, calf thymus, phytohemagglutinin-stimulated human lymphocytes, rat liver, and rabbit bone marrow). The results suggest that (i) no antigenic relationship exists between terminal transferase and DNA polymerase, (ii) common antigenic determinants exist in the DNA polymerases from all mammalian sources, and (iii) multiple forms of DNA polymerase found in mammalian, cells are related by having polypeptide sequences or subunits in common.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
DNA polymerases in parasitic protozoans differ from host enzymes.
L. Chang, E Cheriathundam, E. Mahoney, and A Cerami (1980)
Science 208, 510-511
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Science. ISSN 0036-8075 (print), 1095-9203 (online)