Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 3 March 1972:
Vol. 175. no. 4025, pp. 1006 - 1008
DOI: 10.1126/science.175.4025.1006
Prev | Table of Contents | Next

Articles

H-2 Antigen on Cell Membranes: An Explanation for the Alteration of Distribution by Indirect Labeling Techniques

William C. Davis 1

1 Departments of Veterinary Pathology and Microbiology, Washington State University, Pullman 99163

The distribution of H-2 isoantigens on the plasma membrane of lymphocytes and thymocytes was studied with ferritin-conjugated alloantibody. The spatial arrangement of the membrane components which possess H-2 isoantigens is markedly altered by the attachment of a second antibody, directed toward mouse gamma globulin or ferritin; the second anitibody appears to cause the aggregation of the sensitized H-2 antigens into discrete zones on the cell membrane. Other membrane antigens may be equally affected by indirect labeling techniques and, thus, topographic analysis of the antigenic structure should be done with antibody that is directly conjugated.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Mobility and the Restriction of Mobility of Plasma Membrane Lectin-Binding Components.
G. L. Nicolson and R. Yanagimachi (1974)
Science 184, 1294-1296
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)