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Science 25 February 1972:
Vol. 175. no. 4024, pp. 906 - 908
DOI: 10.1126/science.175.4024.906
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Articles

Modulation of Adenylate Cyclase Activity in Liver and Fat Cell Membranes by Insulin

Gennaro Illiano 1 and Pedro Cuatrecasas 1

1 Department of Medicine and Department of Pharmacology and Experimental Therapeutics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205

Insulin depresses both the activity of adenylate cyclase stimulated by glucagon, epinephrine, and sodium fluoride in liver cell membranes and the activity of adenylate cyclase stimulated by epinephrine and adrenocorticotropin in particulate preparations from homogenates of isolated fat cells. Significant inhibition is detected with very low concentrations (10-11 molar) of insulin but not with unphysiologically high (10-9molar) concentrations of the hormone. These direct effects of insulin on an enzymatic system in broken-cell -preparations suggest a fundamental role of adenylate cyclase activity and of cyclic adenosine monophosphate in the mechanism of action of insulin.


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Somatomedin: Inhibition of Adenylate Cyclase Activity in Subcellular Membranes of Various Tissues.
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Biological Activity of Insulin-Sepharose?.
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