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Science 18 February 1972:
Vol. 175. no. 4023, pp. 768 - 769
DOI: 10.1126/science.175.4023.768
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Articles

1,25-Dihydroxycholecalciferol: A Potent Stimulator of Bone Resorption in Tissue Culture

L. G. Raisz 1, C. L. Trummel 1, M. F. Holick 2, and H. F. Deluca 2

1 Departments of Pharmacology and Toxicology and Dentistry and Dental Research, University of Rochester, Rochester, New York 14642
2 Department of Biochemistry, University of Wisconsin, Madison 53706

1,25-Dihydroxycholecalciferol (DHCC), isolated from kidney homogenates incubated with 25-hydroxycholecalciferol (HCC), stimulated the release of previously incorporated 4545Ca from fetal rat bones in organ culture, at concentrations of 10-10 to 10-8M. The dose response curves for 1,25-DHCC and 25-HCC, the parent compound, are parallel, but 1,25-DHCC is about 100 times as potent on a weight basis. Brief exposure to maximum doses of either agent leads to prolonged bone resorption.


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