Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 11 February 1972:
Vol. 175. no. 4022, pp. 646 - 647
DOI: 10.1126/science.175.4022.646
Prev | Table of Contents | Next

Articles

A Fluorescent Modification of Adenosine Triphosphate with Activity in Enzyme Systems: 1,N6-Ethenoadenosine Triphosphate

John A. Secrist III 1, Jorge R. Barrio 1, and Nelson J. Leonard 1

1 School of Chemical Sciences, University of Illinois, Urbana 61801

A new, highly fluorescent adenosine triphophate (ATP) analog, 1,N6 ethenoadenosine triphosphate, has been synthesized. Its fluorescence properties, including the long fluorescence lifetime and the possibility of detection at very low concentrations, in conjunction with its activity in the representative enzyme systems here reported, make it a valuable probe of enzymic mechanism and structure.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Directed evolution of an orthogonal nucleoside analog kinase via fluorescence-activated cell sorting.
L. Liu, Y. Li, D. Liotta, and S. Lutz (2009)
Nucleic Acids Res. 37, 4472-4481
   Abstract »    Full Text »    PDF »
Analytical Ancestry: "Firsts" in Fluorescent Labeling of Nucleosides, Nucleotides, and Nucleic Acids.
L. J. Kricka and P. Fortina (2009)
Clin. Chem. 55, 670-683
   Abstract »    Full Text »    PDF »
Characterization and use of an unprecedentedly bright and structurally non-perturbing fluorescent DNA base analogue.
P. Sandin, K. Borjesson, H. Li, J. Martensson, T. Brown, L. M. Wilhelmsson, and B. Albinsson (2008)
Nucleic Acids Res. 36, 157-167
   Abstract »    Full Text »    PDF »
Mechanism of active repolarization of inhibitory junction potential in murine colon.
S. A. Baker, V. Mutafova-Yambolieva, K. Monaghan, B. Horowitz, K. M. Sanders, and S. D. Koh (2003)
Am J Physiol Gastrointest Liver Physiol 285, G813-G821
   Abstract »    Full Text »    PDF »
Use of nucleotide analogs by class I and class II CCA-adding enzymes (tRNA nucleotidyltransferase): Deciphering the basis for nucleotide selection.
H. D. CHO, A. K. OYELERE, S. A. STROBEL, and A. M. WEINER (2003)
RNA 9, 970-981
   Abstract »    Full Text »    PDF »
Helicase from Hepatitis C Virus, Energetics of DNA Binding.
M. K. Levin and S. S. Patel (2002)
J. Biol. Chem. 277, 29377-29385
   Abstract »    Full Text »    PDF »
Correlation between the Release of the Sympathetic Neurotransmitter ATP and Soluble Nucleotidases from the Guinea Pig Vas Deferens.
S. Mihaylova-Todorova, L. D. Todorov, and D. P. Westfall (2001)
J. Pharmacol. Exp. Ther. 296, 64-70
   Abstract »    Full Text »
Synthesis and Characterization of Stacked and Quenched Uridine Nucleotide Fluorophores.
G. Dhar and A. Bhaduri (1999)
J. Biol. Chem. 274, 14568-14572
   Abstract »    Full Text »    PDF »
Defined dimensional changes in enzyme cofactors: fluorescent "stretched-out" analogs of adenine nucleotides.
D. Scopes, Barrio JR, and N. Leonard (1977)
Science 195, 296-298
   Abstract »    PDF »
Fluorescent Modification of Adenosine 3',5'-Monophosphate: Spectroscopic Properties and Activity in Enzyme Systems.
J. A. Secrist III, J. R. Barrio, N. J. Leonard, C. Villar-Palasi, and A. G. Gilman (1972)
Science 177, 279-280
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)