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Science 29 October 1971:
Vol. 174. no. 4008, pp. 500 - 503
DOI: 10.1126/science.174.4008.500
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Articles

Buffer Combinations for Mammalian Cell Culture

Harry Eagle 1

1 Albert Einstein College of Medicine, Bronx, New York 10461

The growth and metabolism of cultured mammalian cells are markedly affected by the pH variation in ordinary hicarbonate-buffered media (pH 8.0 to 6.9). Those pH swings can be reduced and the pH of the culture can be stabilized as desired in the range pH 6.4 to 8.3 by appropriate combinations of two or three organic buffers, each at 10 to 15 millimolar, in conjunction with phosphate and bicarbonate. The initial alkalinization in sparse cultures is then minimized, and the metabolic acidificatiotn in 24 hours is usually less than 0.4 pH unit except in heavy cultutres.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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The Effect of Adsorption on the Acid Production of Caries and Noncaries-Producing Streptococci.
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Journal of Dental Research 56, 1193-1200
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Science. ISSN 0036-8075 (print), 1095-9203 (online)