Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 2 July 1971:
Vol. 173. no. 3991, pp. 51 - 54
DOI: 10.1126/science.173.3991.51
Prev | Table of Contents | Next

Articles

1,25-Dihydroxycholecalciferol: Identification of the Proposed Active Form of Vitamin D3 in the Intestine

Anthony W. Norman 1, James F. Myrtle 1, Ronald J. Miogett 1, Henry G. Nowicki 1, Vincent Williams 2, and G. Popjaák 2

1 Department of Biochemistry, University of California, Riverside 92502
2 Department of Biological Chemistry, School of Medicine, University of California, Los Angeles 90024

The major polar metabolite of cholecalciferol (vitamin D3) present in chick intestinal mucosa has been chemically characterized by mass spectrometric analysis to have a molecular formula of C27H4403 and a structure of 1,25-dihydroxycholecalciferol. This compound, which is produced in the kidney from 25-hydroxycholecalciferol, has been previously shown to be from 4 to 13 times as active as cholecalciferol in stimulating intestinal calcium transport. 1,25-Dihydroxycholecalciferol (previously designated metabolite 4B in this (laboratory) probably represents the biologically active form of cholecalciferol in the intestine.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Vitamin D and Human Health: Lessons from Vitamin D Receptor Null Mice.
R. Bouillon, G. Carmeliet, L. Verlinden, E. van Etten, A. Verstuyf, H. F. Luderer, L. Lieben, C. Mathieu, and M. Demay (2008)
Endocr. Rev. 29, 726-776
   Abstract »    Full Text »    PDF »
Vitamin D Receptor: New Assignments for an Already Busy Receptor.
A. W. Norman (2006)
Endocrinology 147, 5542-5548
   Abstract »    Full Text »    PDF »
Structure-Function Analysis of Vitamin D 24-Hydroxylase (CYP24A1) by Site-Directed Mutagenesis: Amino Acid Residues Responsible for Species-Based Difference of CYP24A1 between Humans and Rats.
H. Hamamoto, T. Kusudo, N. Urushino, H. Masuno, K. Yamamoto, S. Yamada, M. Kamakura, M. Ohta, K. Inouye, and T. Sakaki (2006)
Mol. Pharmacol. 70, 120-128
   Abstract »    Full Text »    PDF »
Commentary: Sunlight, vitamin D, and the cancer connection revisited.
K. M Egan (2006)
Int. J. Epidemiol. 35, 227-230
   Full Text »    PDF »
Characterization of recombinant CYP2C11: a vitamin D 25-hydroxylase and 24-hydroxylase.
M. Rahmaniyan, K. Patrick, and N. H. Bell (2005)
Am J Physiol Endocrinol Metab 288, E753-E760
   Abstract »    Full Text »    PDF »
Targeted ablation of the 25-hydroxyvitamin D 1alpha -hydroxylase enzyme: Evidence for skeletal, reproductive, and immune dysfunction.
D. K. Panda, D. Miao, M. L. Tremblay, J. Sirois, R. Farookhi, G. N. Hendy, and D. Goltzman (2001)
PNAS 98, 7498-7503
   Abstract »    Full Text »    PDF »
Differential Use of Transcription Activation Function 2 Domain of the Vitamin D Receptor by 1,25-Dihydroxyvitamin D3 and Its A Ring-Modified Analogs.
S. Peleg, C. Nguyen, B. T. Woodard, J.-K. Lee, and G. H. Posner (1998)
Mol. Endocrinol. 12, 525-535
   Abstract »    Full Text »
Calcinogenic factor in Solanum malacoxylon: evidence that it is 1,25-dihydroxyvitamin D3-glycoside.
R. Wasserman, J. Henion, M. Haussler, and T. McCain (1976)
Science 194, 853-855
   Abstract »    PDF »
Regulation of serum 1alpha,25-dihydroxyvitamin D3 by calcium and phosphate in the rat.
M. Hughes, P. Brumbaugh, M. Hussler, J. Wergedal, and D. Baylink (1975)
Science 190, 578-580
   Abstract »    PDF »
Pharmacology and Therapeutics of Bone.
W.C. Sturtridge (1975)
Journal of Dental Research 54, B78-B86
   PDF »
Radioreceptor Assay for 1agr,25-Dihydroxyvitamin D3.
P. F. Brumbaugh, D. H. Haussler, R. Bressler, and M. R. Haussler (1974)
Science 183, 1089-1091
   Abstract »    PDF »
Intestinal Absorption of Calcium.
L. V. Avioli (1972)
Arch Intern Med 129, 345-355
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)