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Science 7 May 1971:
Vol. 172. no. 3983, pp. 577 - 579
DOI: 10.1126/science.172.3983.577
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Articles

Actinomycin D: Renewed RNA Synthesis after Removal from Mammalian Cells

Ann Schluederberg 1, Robert C. Hendel 1, and Surina Chavanich 1

1 Department of Epidemiology and Public Health, Yale University Medical School, New Haven, Connecticut 06510

Suppressed RNA synthesis exceeded control rates 2 hours after removal of actinomycin from serial lines of green monkey kidney cells in exponentially dividing monolayer cultures. Enhanced synthesis was directly related to the dose of actinomycin, and release from inhibition was temperature dependent. A broad range of RNA sizes was made after removal of drug, but cytoplasmic and nucleolar sedimentation profiles were abnormal for at least 20 hours.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)