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Science 23 April 1971:
Vol. 172. no. 3981, pp. 385 - 387
DOI: 10.1126/science.172.3981.385
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Articles

Nucleotide Sequence of the "Denaturable" Leucine Transfer RNA from Yeast

Steven Kowalski 1, Tetsuo Yamane 1, and Jacques R. Fresco 1

1 Department of Biochemical Sciences, Frick Chemical Laboratory, Princeton University, Princeton, New Jersey, and Medical Research Council Laboratory for Molecular Biology, Cambridge, England

The nucleotide sequence of " denaturable"leucine acceptor transfer RNA (tRNALeu3) from baker's yeast was determined on 32P-labeled material. The molecule is 85 nucleotides long and can be folded into the "cloverleaf" model for secondary structure. The basis on which the sequence was deduced from the products of complete enzymatic digestion, prior to its unambiguous determination, is presented.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Preparation of Highly Labeled [32P]Nucleic Acids from Yeast; Isolation of "Denaturable" Leucine Acceptor Transfer RNA.
S. Kowalski and J. R. Fresco (1971)
Science 172, 384-385
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Science. ISSN 0036-8075 (print), 1095-9203 (online)