Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 15 January 1971:
Vol. 171. no. 3967, pp. 187 - 189
DOI: 10.1126/science.171.3967.187
Prev | Table of Contents | Next

Articles

Nerve Trophic Function: In vitro Assay of Effects of Nerve Tissue on Muscle Cholinesterase Activity

Thomas L. Lentz 1

1 Department of Anatomy, Yale University School of Medicine, New Haven, Connecticut 06510

The effects of tissue explants and nerve homogenates on cholinesterase activity of muscle of the newt Triturus in organ culture were measured. Sensory ganglia, ganglia separated from muscle by a Millipore filter, spinal cord, liver, and nerve homogenates produced greater activity of muscle cholinesterase than occurred in untreated muscle cultured for the same period of time. Boiled ganglia, kidney, oviduct, and spleen were ineffective. This procedure serves as a convenient bioassay for a neurotrophic process and indicates that the trophic effect is mediated by a diffusible chemical substance produced by nerves.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
A soluble neuronal factor alters contractile function of ventricular myocytes without effect on troponin T isoform expression.
S. H. Green, R. M. Glover, P. A. Krumm, T. R. Winters, and D. L. Atkins (1999)
Cardiovasc Res 41, 200-211
   Abstract »    Full Text »    PDF »
Substances moved by axonal transport and released by nerve stimulation have an innervation-like effect on muscle.
S. Younkin, R. Brett, B Davey, and L Younkin (1978)
Science 200, 1292-1295
   Abstract »    PDF »
Neurotrophic protein regulates muscle acetylcholinesterase in culture.
T. Oh and G. Markelonis (1978)
Science 200, 337-339
   Abstract »    PDF »
Choline Acetyltransferase Activity Is Increased in Combined Cultures of Spinal Cord and Muscle Cells from Mice.
E. L. Giller Jr., B. K. Schrier, A. Shainberg, H. R. Fisk, and P. G. Nelson (1973)
Science 182, 588-589
   Abstract »    PDF »
Hyperinnervation of Skeletal Muscle Fibers: Dependence on Muscle Activity.
J. K. S. Jansen, T. Lomo, K. Nicolaysen, and R. H. Westgaard (1973)
Science 181, 559-561
   Abstract »    PDF »
Neurotrophic Effect on Isolated Chick Embryo Muscle in Culture.
T. H. Oh, D. D. Johnson, and S. U. Kim (1972)
Science 178, 1298-1300
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)