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Science 6 November 1970:
Vol. 170. no. 3958, pp. 639 - 641
DOI: 10.1126/science.170.3958.639
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Articles

Localization of 5S RNA Genes on Drosophila Chromosomes by RNA-DNA Hybridization

D. E. Wimber 1 and D. M. Steffensen 1

1 Department of Botany, University of Illinois, Urbana 61801

[3H]RNA with a high specific activity was prepared from larvae of Drosophila melanogaster grown 4 days in contact with [3H]uridine. Purified tritiated 5S RNA was annealed to the DNA of polytene chromosomes, which had been denatured in formamide. The 5S RNA genes are placed within the region 56E-F of the right arm of chromosome 2. This localization was determined from autoradiographs, where the radioactivity from hybrids of [3H]RNA and DNA was confined to the 56E-F segment.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)