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Science 23 October 1970:
Vol. 170. no. 3956, pp. 441 - 445
DOI: 10.1126/science.170.3956.441
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Articles

Bimodal Sedimenting Zones Due to Ligand-Mediated Interactions

John R. Cann 1 and Walter B. Goad 2

1 Department of Biophysics, University of Colorado Medical Center, Denver 80220
2 University of California Los Alamos Scientific Laboratory, Los Alamos, New Mexico 87544

Ligand-mediated association-dissociation reactions can give rise to band sedimentation patterns showing bimodal bands despite instantaneous establishment of equilibrium. Weaker interactions result in unimodal bands whose sedimentation coefficients decrease with time of sedimentation and in characteristic patterns of total ligand. The implications of these results for fundamental investigations of protein interactions and for conventional analytical applications of zone sedimentation and molecular sieve chromatography are considered.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)