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Science 16 October 1970:
Vol. 170. no. 3955, pp. 332 - 333
DOI: 10.1126/science.170.3955.332
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Articles

Hemagglutination Assay for Antigen and Antibody Associated with Viral Hepatitis

G. N. Vyas 1 and N. R. Shulman 2

1 Department of Clinical Pathology and Laboratory Medicine, University of California School of Medicine, San Francisco 94122
2 Clinical Hematology Branch, National Institute of Arthritis and Metabolic Diseases, Bethesda, Maryland 20014

Hemagglutination assays are described for measuring hepatitis-associated Australia antigen and antibody. Red cells coated with isolated antigen, with chromic chloride as a coupling agent, are used for detection of antibodies. Detection of the antigen in serums depends on inhibition of hemagglutination. The test has the sensitivity and rapidity of the best tests available, is simpler to perform, and lends itself to large-scale screening of blood donors.


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JAMA 227, 1042-1043
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Hepatitis B Transmission in a Family.
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JAMA 227, 1043-1044
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JAMA 227, 901-906
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Antibody Responses in Viral Hepatitis, Type B.
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JAMA 223, 1005-1008
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Australia Antigen (Hepatitis B Antigen): A Conformational Antigen Dependent on Disulfide Bonds.
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Posttransfusion Hepatitis After Exclusion of Commercial and Hepatitis-B Antigen-Positive Donors.
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Ann Intern Med 77, 691-699
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Coincidental Australia Antigenemia in Primary Biliary Cirrhosis.
W. C. MADDREY, S. SAITO, N. R. SHULMAN, and G. KLATSKIN (1972)
Ann Intern Med 76, 705-709
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Epidemiology of Infections With Type B Virus.
R. W. McCollum (1972)
Arch Pediatr Adolesc Med 123, 364-367
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Australia Antigen and Polyarteritis Nodosa.
C. G. Trepo, J. Thivolet, and A. M. Prince (1972)
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Immune Response in Patients Who Received Blood Containing Serum Hepatitis Antigen.
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Arch Pediatr Adolesc Med 123, 415-418
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A Prospective Study of Posttransfusion Hepatitis: The Role of Australia Antigen.
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Hepatoma and HAA.
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Viral Hepatitis, Type B (MS-2 Strain) Studies On Active Immunization.
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Australia Antigen Ecology.
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Ann Intern Med 75, 130-132
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