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Science 10 July 1970:
Vol. 169. no. 3941, pp. 204 - 205
DOI: 10.1126/science.169.3941.204

Articles

Automated Continuous Culture of Mammalian Cells in Suspension

Carl Peraino 1, Silvia Bacchetti 1, and William J. Eisler 1

1 Division of Biological and Medical Research, Argonne National Laboratory, Argonne, Illinois 60439

A system has been developed for the continuous culture of mammalian cells in suspension. The system maintains constant cell concentrations (monitored as the degree of light scattering) over a wide range of previously selected values by automatic additions of known amounts of medium and simultaneous withdrawals of equal volumes of cell suspension.





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Science. ISSN 0036-8075 (print), 1095-9203 (online)