Automated Continuous Culture of Mammalian Cells in Suspension
Carl Peraino 1,
Silvia Bacchetti 1, and
William J. Eisler 1
1 Division of Biological and Medical Research, Argonne National Laboratory, Argonne, Illinois 60439
A system has been developed for the continuous culture of mammalian cells in suspension. The system maintains constant cell concentrations (monitored as the degree of light scattering) over a wide range of previously selected values by automatic additions of known amounts of medium and simultaneous withdrawals of equal volumes of cell suspension.