Equilibrium Density-Gradient Procedure for Selection of Synchronous Cells from Asynchronous Cultures
Thomas O. Sitz 1,
Alan B. Kent 1,
Harold A. Hopkins 1, and
Robert R. Schmidt 1
1 Department of Biochemistry and Nutrition, Virginia Polytechnic Institute, Blacksburg 24061
A reproducible isopycnic technique, in which linear density gradients of Ficoll are used, was developed for selecting synchronous daughter cells from asynchronous cultures of Chlorella for biochemical studies of the cell cycle.