Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 29 May 1970:
Vol. 168. no. 3935, pp. 1111 - 1112
DOI: 10.1126/science.168.3935.1111
Prev | Table of Contents | Next

Articles

Insulin Release from Isolated Human Fetal Pancreatic Islets

A. Espinosa De Los Monteros 1, S. G. Driscoll 1, and J. Steinke 1

1 E. P. Joslin Research Laboratory, Harvard Medical School, 170 Pilgrim Road, Boston, Massachusetts 02215

Pancreases were obtained from five human fetuses 12 to 16 weeks old. The islets of Langerhans were isolated with collagenase, and then incubated with buffer, glucose, tolbutamide, or glucagon added to the medium. The insulin released into the medium was measured by immunoassay. Glucagon produced the only significant increase above base line; glucose and tolbutamide failed to enhance secretion of insulin. The data suggest that isolated human fetal islets of this gestational age develop responsiveness to glucagon earlier than to glucose or tolbutamide.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Nuclear Factor-{kappa}B Activity in {beta}-Cells Is Required for Glucose-Stimulated Insulin Secretion.
S. Norlin, U. Ahlgren, and H. Edlund (2005)
Diabetes 54, 125-132
   Abstract »    Full Text »    PDF »
Complete reversal of experimental diabetes mellitus in rats by a single fetal pancreas.
Y. Mullen, W. Clark, I. Molnar, and J Brown (1977)
Science 195, 68-70
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)