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Science 24 April 1970:
Vol. 168. no. 3930, pp. 487 - 489
DOI: 10.1126/science.168.3930.487
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Articles

Induction and Isolation of Auxotrophic Mutants in Somatic Cell Cultures of Nicotiana tabacum

Peter S. Carlson 1

1 Department of Biology, Yale University, New Haven, Connecticut 06520

A method for the selection of auxotrophic mutants from somatic cell cultures of Nicotiana tabacum depends upon the incorporation of 5-bromodeoxyuridine into the DNA of wild-type cells and upon its lack of incorporation into the DNA of auxotrophic cells. There may be more than one functional copy of essential genes in the haploid genome of Nicotiana tabacum.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Isolation of Agronomically Useful Mutants from Plant Cell Cultures.
R. S. Chaleff (1983)
Science 219, 676-682
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Methionine Sulfoximine-Resistant Mutants of Tobacco.
P. S. Carlson (1973)
Science 180, 1366-1368
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Science. ISSN 0036-8075 (print), 1095-9203 (online)