Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 27 March 1970:
Vol. 167. no. 3926, pp. 1736 - 1738
DOI: 10.1126/science.167.3926.1736
Prev | Table of Contents | Next

Articles

Induction of Glutamine Synthetase in Embryonic Retina: Its Dependence on Cell Interactions

John E. Morris 1 and A. A. Moscona 1

1 Department of Biology, University of Chicago, Chicago, Illiniois 60637

A relation between enzyme induction in embryonic cells and cellular organization is indicated by the finding that the levels of glutamine synthetase induced by hydrocortisone in the embryonic neural retina in vitro are dependent on the associations between the retina cells. Intact retina tissue, aggregates of dissociated cells, and cells in monolayer culture showed a decreasing response, in this order, to glutamine synthetase induction. With time of culture, the enzyme activity continued to rise in the intact retina and in cell aggregates, but activity declined in monolayer cultures even though the inducer was continuously present. Dispersed cells cultured in monolayer without the inducer showed after 24 hours a loss of inducibility which could not be reversed by reaggregating such modified cells but could be prevented by maintaining the freshly dispersed cells at a low temperature.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Development of Sensitivity to Tetrodotoxin in Beating Chick Embryo Hearts, Single Cells, and Aggregates.
T. F. McDonald, H. G. Sachs, and R. L. DeHaan (1972)
Science 176, 1248-1250
   Abstract »    PDF »
Glutamine Synthetase Activity in Human Retinal Tissue: Enzyme Activity in the Normal Retina and Cortisol Induction in Cultured Cells Derived From Retinoblastoma.
L. Reif-Lehrer (1971)
Arch Ophthalmol 86, 72-76
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)