Isolation and Zonal Fractionation of Metaphase Chromosomes from Human Diploid Cells
Edward L. Schneider 1 and
Norman P. Salzman 1
1 Laboratory of Biology of Viruses, National Institutes of Health, Bethesda, Maryland 20014
A simple method for isolating large quantities of human metaphase chromosomes has been developed. Fractionation of chromosomes from peripheral lymphocytes on a large scale is accomplished by velocity sedimentation in an A-XII zonal rotor.