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Science 20 February 1970:
Vol. 167. no. 3921, pp. 1141 - 1143
DOI: 10.1126/science.167.3921.1141-a

Articles

Isolation and Zonal Fractionation of Metaphase Chromosomes from Human Diploid Cells

Edward L. Schneider 1 and Norman P. Salzman 1

1 Laboratory of Biology of Viruses, National Institutes of Health, Bethesda, Maryland 20014

A simple method for isolating large quantities of human metaphase chromosomes has been developed. Fractionation of chromosomes from peripheral lymphocytes on a large scale is accomplished by velocity sedimentation in an A-XII zonal rotor.





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Science. ISSN 0036-8075 (print), 1095-9203 (online)