Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 9 January 1970:
Vol. 167. no. 3915, pp. 186 - 188
DOI: 10.1126/science.167.3915.186
Prev | Table of Contents | Next

Articles

Immunoglobulin Production: Method for Quantitatively Detecting Variant Myeloma Cells

Philip Coffino 1, Reuven Laskov 1, and Matthew D. Scharff 2

1 Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461
2 Departments of Cell Biology and Medicine, Albert Einstein College of Medicine

Mouse myeloma cells in continuous culture were cloned in soft agar. The clones were assayed for their ability to synthesize heavy and light chains of gamma globulin by immunoprecipitation directly in the agar. A minor population secreting only light chains was identified. The two cell types were recloned and a low incidence of conversion of 7S to light-chain production was demonstrated. This technique can be used to isolate rare variants of cells secreting specific macromolecules.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Regulation of Immunoglobulin Expression in Mouse Myeloma Cells.
D. H. Margulies, W. Cieplinski, B. Dharmgrongartama, M. L. Gefter, S. L. Morrison, T. Kelly, and M. D. Scharff (1977)
Cold Spring Harb Symp Quant Biol 41, 781-791
   Abstract »    PDF »
Mutations in Immunoglobulin-Producing Moose Myeloma Cells.
R. Baumal, B. K. Birshtein, P. Coffino, and M. D. Scharff (1973)
Science 182, 164-166
   Abstract »    PDF »
Quantitative Aspects of Plasma Membrane-Associated Immunoglobulin in Clones of Diploid Human Lymphocytes.
R. A. Lerner, P. J. McConahey, and F. J. Dixon (1971)
Science 173, 60-62
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)