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Science 9 January 1970:
Vol. 167. no. 3915, pp. 179 - 181
DOI: 10.1126/science.167.3915.179

Articles

Absorption of Proteins and Peptides in the Far Ultraviolet

A. H. Woods 1 and P. R. O'Bar 1

1 Veterans Administration Hospital, Tucson, Arizona, and Department of Medicine, College of Medicine, University of Arizona, Tucson

An absorption peak for the peptide bond at 187 nanometers has been confirmed; a protein assay at this wavelength allows quantitation of proteins in aqueous solution at concentrations between 0.1 and 25 micrograms per milliliter. Assays are conducted at 6°C to take advantage of the reduction of end-absorption of water with temperature.





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Science. ISSN 0036-8075 (print), 1095-9203 (online)