Absorption of Proteins and Peptides in the Far Ultraviolet
A. H. Woods 1 and
P. R. O'Bar 1
1 Veterans Administration Hospital, Tucson, Arizona, and Department of Medicine, College of Medicine, University of Arizona, Tucson
An absorption peak for the peptide bond at 187 nanometers has been confirmed; a protein assay at this wavelength allows quantitation of proteins in aqueous solution at concentrations between 0.1 and 25 micrograms per milliliter. Assays are conducted at 6°C to take advantage of the reduction of end-absorption of water with temperature.