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Science 2 January 1970:
Vol. 167. no. 3914, pp. 69 - 72
DOI: 10.1126/science.167.3914.69
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Articles

Duplicate Plating of Immune Cell Products: Analysis of Globulin Class Secretion by Single Cells

Bruce Merchant 1 and Zacharie Brahmi 2

1 Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20014
2 Department of Botany, Howard University, Washington, D.C.

Antibodies secreted by individual immune cells were collected focally in very thin "original" and "imprint" layers of agar containing the target antigen, sheep erythrocytes. Identical treatment of both layers led to mirror image patterns of hemolytic plaques. Development of one layer for immunoglobulin M hemolysins and the other for immunoglobulin G hemolysins produced unrelated plaque patterns indicating that few, if any, cells simultaneously release substantial amounts of both ggrM and ggrG antibodies.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Density Gradient Separation of Marrow Cells Restricted for Antibody Class.
H. C. Miller and G. Cudkowicz (1971)
Science 171, 913-915
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