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Science 1 August 1969:
Vol. 165. no. 3892, pp. 504 - 506
DOI: 10.1126/science.165.3892.504
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Articles

Noncovalent Binding of a Spin-Labeled Inhibitor to Ribonuclease

Gordon C. K. Roberts 1, John Hannah 1, and Oleg Jardetzky 1

1 Departments of Biophysics and Synthetic Chemistry, Merck Sharp & Dohme Research Laboratories, Rahway, New Jersey 07065

The stable free radical 2,2,6, 6-tetramethyl-4-hydroxypiperidin-1-oxyl monophosphate has been synthesized; it binds to ribonuclease. The selective changes in the nuclear magnetic resonance spectrum of the enzyme produced by the free radical make it possible to define qualitatively the region of the enzyme to which it binds. The radical appears to occupy a site similar to that to which inorganic phosphate binds which is close to or within the active site of the enzyme.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)