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Science 4 July 1969:
Vol. 165. no. 3888, pp. 75 - 76
DOI: 10.1126/science.165.3888.75
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Articles

Actinomycin Binding to DNA: Inability of a DNA Containing Guanine To Bind Actinomycin D

Robert D. Wells 1

1 Department of Biochemistry, University of Wisconsin, Madison 53706

Polydeoxy (adenylyl-thymidylyl-cytidylyl)* polydeoxy(guanylyl-adenylyl-thymidylyl), a double-stranded DNA polmer of high molecular weight containing 33 percent guanine plus cytosine, binds little or no actinomycin D, as measured by five different techniques. In contrast, the sequence isomer of this DNA, polydeoxy (thymidylyl-adenylyl-cytidylyl)-polydeoxy(guanylyl-thymidylyl-adenylyl), does bind the antibiotic. Thus, the presence of guanine in a DNA is not a sufficient requisite for the binding of actinomycin D.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Discovery of the Role of Non-B DNA Structures in Mutagenesis and Human Genomic Disorders.
R. D. Wells (2009)
J. Biol. Chem. 284, 8997-9009
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Cloning, Characterization, and Properties of Seven Triplet Repeat DNA Sequences.
K. Ohshima, S. Kang, J. E. Larson, and R. D. Wells (1996)
J. Biol. Chem. 271, 16773-16783
   Abstract »    Full Text »    PDF »


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