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Science 27 June 1969:
Vol. 164. no. 3887, pp. 1532 - 1534
DOI: 10.1126/science.164.3887.1532
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Articles

Immunoglobulins G, A, and M Determined in Single Cells from Human Tonsil

David Gitlin 1 and Teruo Sasaki 1

1 Department of Pediatrics, Children's Hospital of Pittsburgh, Pittsburgh, Pennsylvania

The analysis of specific proteins in single cells is described and applied to the determination of immunoglobulins G, A, and M (ggrG, ggrA, and ggrM) in lymphoid cells of the human tonsil. The number of cells containing ggrA or ggrM was much less than the number of cells containing ggrG, but the average cell content of ggrA or ggrM was much greater than the average cell content of ggrG. Similarly, in one child where relatively few cells containing ggrG were found, the average cell content of ggrG in those cells was higher than that in the other children. The limit of sensitivity of the method for each of the three immunoglobulins with the antiserums used was less than 10-14 gram of the specific protein.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Immunoglobulin Production by Human Lymphocytoid Lines and Clones: Absence of Genic Exclusion.
A. D. Bloom, K. W. Choi, and B. J. Lamb (1971)
Science 172, 382-383
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Science. ISSN 0036-8075 (print), 1095-9203 (online)