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Science 13 June 1969:
Vol. 164. no. 3885, pp. 1292 - 1293
DOI: 10.1126/science.164.3885.1292
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Articles

Human Ooeytes: Maturation in Chemically Defined Media

Joseph F. Kennedy 1 and Roger P. Donahue 2

1 Division of Population Dynamnics and Department of Gynecology and Obstetrics, Johns Hopkins University, Baltimore, Maryland 21205
2 Division of Medical Genetics, Department of Medicine

Human oocytes from ovarian follicles resume meiosis in F10, a defined medium, in numbers comparable to that obtained in medium containing serum. Several simple Krebs-Ringer media also support maturation, which suggests a similarity of nutritional requirements between human and mouse oocytes. Fewer oocytes reach metaphase II when the investing follicular cells are removed prior to culture.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Assessment of a new in vitro maturation system for mouse and human cumulus-enclosed oocytes: three-dimensional prematuration culture in the presence of a phosphodiesterase 3-inhibitor.
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Prematuration of human denuded oocytes in a three-dimensional co-culture system: effects on meiosis progression and developmental competence.
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Nuclear and cytoplasmic maturation of in vitro matured human oocytes after temporary nuclear arrest by phosphodiesterase 3-inhibitor.
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Production of Progesterone from De Novo-Synthesized Cholesterol in Cumulus Cells and Its Physiological Role During Meiotic Resumption of Porcine Oocytes.
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Effects of Sodium Pyruvate in Nonserum Maturation Medium on Maturation, Fertilization, and Subsequent Development of Bovine Oocytes With or Without Cumulus Cells.
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