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Science 9 May 1969:
Vol. 164. no. 3880, pp. 721 - 722
DOI: 10.1126/science.164.3880.721
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Articles

Deoxyribonucleic Acid Methylase Activity in Pea Seedlings

Frantisek Kalousek 1 and N. Ronald Morris 2

1 Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, Prague 6, Czechoslovakia
2 Department of Pharmacology, Rutgers Medical School, New Brunswick, New Jersey 08903

Deoxyribonucleic acid methylase activity has been detected in a preparation of disrupted nuclei prepared from pea seedlings. S-Adenosyl-L-methionine acted as a donor of methyl groups, and the product of the reaction was identified as 5-methylcytosine. The reaction had a sharp temperature optimum at about 30°C and was unusual in that the DNA methylase was able to methylate DNA in the crude extract.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)